Metodo LAMP como alternativa diagnostica para la deteccion del virus SARS-CoV-2

Summary Introduction: The SARS-CoV-2 coronavirus, that causes the COVID-19 disease, has become a global public health problem that requires the implementation of rapid and sensitive diagnostic tests. Aim(s): To evaluate and compare the sensitivity of LAMP assay to a standard method and use RT-LAMP for the diagnosis of SARS-CoV-2 in clinical samples from Colombian patients. Method(s): A descriptive and cross-sectional study was conducted. A total of 25 nasopharyngeal swab samples including negative and positive samples for SARS-CoV-2 were analyzed, through the RT-LAMP method compared to the RT-qPCR assay. Result(s): LAMP method detected ~18 copies of the N gene, in 30 min, evidenced a detection limit similar to the standard method, in a shorter time and a concordance in RT-LAMP of 100% with the results. Conclusion(s): RT-LAMP is a sensitive, specific, and rapid method that can be used as a diagnostic aid of COVID-19 disease.Copyright © 2021. All Rights Reserved.

BASELINE AND TEMPORAL DIFFERENCES IN PROTEOMICS FOLLOWING SARS-CoV-2 INFECTION IN PWH

Background: COVID-19 may be more severe in persons with HIV (PWH). However, underlying biological mechanisms associated with the development of COVID-19 and its clinical severity among antiretroviral therapy (ART) treated PWH are largely unknown. Therefore, we wished to evaluate temporal changes in plasma proteins following SARS-CoV-2 infection and identify pre-infection proteomic markers associated with future COVID-19. Method(s): We analyzed the data of clinical, antibody-confirmed COVID-19 ARTtreated PWH from the global Randomized Trial to Prevent Vascular Events in HIV (REPRIEVE). Individuals were matched on geographic region, age, and sample timing to antibody-negative controls. For cases and controls, pre-COVID-19 pandemic specimens were obtained prior to January 2020 to assess temporal changes and baseline differences in protein expression in relationship to COVID-19 severity, using mixed effects models adjusted for false-discovery rate. Result(s): We compared 257 unique plasma proteins (Olink Proteomics) in 94 COVID-19 antibody-confirmed clinical cases and 113 matched antibody-negative controls, excluding COVID-19 vaccinated participants (median age 50 years, 73% male). 40% of cases were characterized as mild;60% moderate to severe. Median time from COVID-19 infection to follow-up sampling was 4 months. Temporal changes in protein expression differed based on COVID-19 disease severity. Among moderate to severe cases vs. controls, NOS3 increased, whereas ANG, CASP-8, CD5, GZMH, GZMB, ITGB2, and KLRD1 decreased. Higher baseline circulating concentrations of granzymes A, B and H (GZMA, GZMB and GZMH) were associated with the future development of moderate-severe COVID-19 in PWH and were related to immune function, including CD4, CD8 and the CD4/ CD8 ratio. Conclusion(s): We identified temporal changes in novel proteins in closely linked inflammatory, immune, and fibrotic pathways which may relate to COVID-19-related morbidity among ART-treated PWH. Further, we identified key granzyme proteins, serine proteases expressed by cytotoxic T lymphocytes and NK cells in response to foreign antigens, associated with future COVID-19 in PWH. Our results provide unique insights into the biological susceptibility and responses to COVID-19 infection in PWH. (Figure Presented).

Fruit characterization and plant quality of Spanish cedar (Cedrela odorata L.) during the early nursery stage

Objective: The aim of this study was to characterize Spanish cedar (Cedrela odorata L.) fruits and seeds, and to evaluate the effects of provenance, substrata, and fertilizer on germination and plant quality in nursery. Design/Methodology/Approach: The fruits and seeds from Spanish cedars were collected in three different towns: C-32 (Francisco Trujillo Gurria), C-40 (Ernesto Aguirre Colorado), and C-41 (Carlos A. Madrazo). Those towns are in Plan Chontalpa, Tabasco, Mexico. After they were harvested, the samples were morphometrically characterized. Seed production efficiency (SPE), germination (%), and Dickson Quality Index (DQI) were estimated. Two completely randomized experimental designs with factorial arrangement of treatments were used. The factors were the provenance, four or three substrata, and two levels of GreenfoolR 600 fertilizer. Results: Fruits of 3.46 cm in length and 1.81 cm in width were collected;the mean number of seeds per fruit was 49.36. Seed production efficiency ranged from 48.1% to 52.72%, with 32.86% germination. The seeds from Town C-41 obtained the highest germination percentage, with the use of black soil:sand as substrate. The plants fertilized and developed in black soil:sand substrate obtained a higher DQI. Study Limitations/Implications: The activity restriction caused by the Sars-Cov-2 pandemic was the main limitation. The lower number of producers and plantations from Town C-32 was the implication. Findings/Conclusions: Fruit and seed characteristics were different among provenances. The provenance impacted on seed germination, but not on the plant quality index (DQI). Instead, the substrate and fertilizer impacted on the DQI.

Noise pollution in Andalusian cities. Differences and similarities in noise perception before and during confinement COVID-19

Noise pollution is one of the main current environmental problems, and specifically in Andalusia within them, excess noise ranks sixth. Through this work, the aim is to find out what the acoustic perception of Andalusian citizens is, both before and during the development of the COVID-19 pandemic. To find out how this type of pollution affects the population, a quantitative methodology has been used, through individual surveys, carrying out a total of 1152 questionnaires to the Andalusian population residing in cities with more than 40 000 inhabitants. One of the research findings has been that almost three-quarters of the population believes that they live in a very or quite noisy city. We have also detected that the main sources of noise for the Andalusian region are those produced by traffic, speaking loudly, works on public roads and renovations by neighbors. These sources of noise remained in the same order of importance during the confinement stage, although there was a decrease in their incidence, due to the inactivity caused by the pandemic in all aspects of daily life.

Sensationalism vs. information during COVID-19 in Ecuador: A framing theory-based view

The different frames used in the COVID-19 coverage in Ecuador by the national and international press were analyzed through their Twitter accounts. This allowed the examination of which aspects of the pandemic were given greater prominence by each newspaper. The study was based on five generic frames, between 17 March and 16 April 2020. The exploration of these frames was carried out by means of an intersubjective content analysis, using four previously trained encoders. The dominant frames for COVID-19 in Ecuador varied according to the media group studied. 1) The concept of heterogeneity was confirmed within framing theory, according to which a single reality, understood as a textual and visual construct, is addressed in multiple ways by the selection of certain aspects that are given greater emphasis or priority;2) international newspapers and, to a lesser extent, the national press showed their propensity to "internalize" the dynamic of sensationalism;and 3) there are discrepancies in the interests of the readerships with respect to the dominant frames in the media. © 2021, IGI Global.

Referral Criteria for Asthma: An Updated Consensus

Asthma is one of the most prevalent chronic diseases in Spain. In 2019, the Spanish Society of Allergology and Clinical Immunology (SEAIC), the Spanish Society of Primary Care Physicians (SEMERGEN), the Spanish Society of Family and Community Medicine (semFYC), the Spanish Society of General and Family Physicians (SEMG) and the Spanish Society of Pulmonology and Thoracic Surgery (SEPAR) drafted a document laying down the criteria for referral and action guidelines in the diagnosis, control and monitoring of the asthmatic patient to facilitate ongoing care and improved attention in every setting. The new circumstances derived from the Covid-19 pandemic have demanded that some of the recommendations of the previous edition be updated and adapted to the new healthcare situation. © 2021 Sociedad Española de Neumología y Cirugía Torácica (SEPAR)

Clinical profile of patients admitted in an acute psychiatric ward before and during the COVID-19 lockdown

IntroductionThe COVID-19 pandemic entailed several changes in health and medical assistance, economy, and lifestyle. In the Acute Psychiatric Ward of the Hospital Clínic of Barcelona, the implementation of restrictive measures was necessary in order to ensure patients’ safety.ObjectivesTo compare clinical profiles and course of hospitalization of patients admitted before and during the COVID-19 lockdown in our Acute Psychiatric Ward.MethodsAll patients admitted from January 7th to February 25th and from March 19th to May 7th of 2020 in the Acute Psychiatric Hospitalization Unit of Hospital Clínic of Barcelona, Spain, were retrospectively included for analysis and divided into two groups according to the period when they were admitted. Statistical analyses were performed using SPSS, 23.0 version.ResultsA total of 117 inpatients were included (73 admitted before lockdown and 44 during lockdown), being 50.4% male, with a mean age of 42.4 (SD 15.73). Patients from the first group presented a significantly higher proportion of antidepressants prescription at discharge (p<0.05) and more substance use disorders (p<0.05). Regarding the lockdown group, 51% of patients manifested COVID-19-related stress. Time of hospitalization was significantly lower in the lockdown group (p<0.05), even though a significantly higher proportion of patients were discharged at home (p<0.05) compared with the first group.ConclusionsThe situation of lockdown led to a series of changes in our unit and also in the profile of patients admitted, having shorter admissions, lower prescription of antidepressants, and often COVID-related stress. These differences should be considered in future situations in which restrictive measures may be necessary.DisclosureNo significant relationships.

Criminal behavior in Medellin in times of the pandemic crisis, a spatio-temporal model

This paper aimed to identify the effect that the implementation of preventive mandatory social isolation had on the dynamics of security and coexistence in Medellin, Colombia. Using a Bayesian structural time-series model, the weight of this preventive measure in the variation of the indicators was calculated: homicides, forced intra-urban displacement, robbery, motorcycles theft, car theft, sexual crimes, family violence, fights, and personal injuries. The results showed this measure had a direct and significant impact on the behavior of the indicators considered;there were increases between 9.8% and 173%, and decreases between 22.9% and 85%, compared to the projections without this measure.

The covid-19 pandemic according to the international media: Ecuador’s case through framing theory on twitter

The research exposes through Framing Theory the different approaches of the national and international press regarding the terrible events that occurred in Ecuador during the so called “Emergency of Guayaquil” between the end of March and the beginning of April 2020, being the hardest moments suffered under the Covid-19 pandemic. Taking as a basis the proposal of generic frames (conflict, consequences, human interest, morality and responsibility) in the Semetko and Valkenburg scale applied to Twitter, the existence of two very different news constructs between the national and international press is evidenced. While in the national press the frame of consequence predominates through a context of high demand for information from audiences to monitor the development of events, the international press emphasized the frame of human interest in the face of need for morbid scenes and sensationalism. © 2021, Associacao Iberica de Sistemas e Tecnologias de Informacao. All rights reserved.

Incubation period of COVID-19: A systematic review and meta-analysis.

BACKGROUND AND OBJECTIVE: The incubation period of COVID-19 helps to determine the optimal duration of the quarantine and inform predictive models of incidence curves. Several emerging studies have produced varying results; this systematic review aims to provide a more accurate estimate of the incubation period of COVID-19. METHODS: For this systematic review, a literature search was conducted using Pubmed, Scopus/EMBASE, and the Cochrane Library databases, covering all observational and experimental studies reporting the incubation period and published from 1 January 2020 to 21 March 2020.We estimated the mean and 95th percentile of the incubation period using meta-analysis, taking into account between-study heterogeneity, and the analysis with moderator variables. RESULTS: We included seven studies (n=792) in the meta-analysis. The heterogeneity (I2 83.0%, p<0.001) was significantly decreased when we included the study quality and the statistical model used as moderator variables (I2 15%). The mean incubation period ranged from 5.6 (95% CI: 5.2-6.0) to 6.7 days (95% CI: 6.0-7.4) according to the statistical model. The 95th percentile was 12.5 days when the mean age of patients was 60 years, increasing 1 day for every 10 years. CONCLUSION: Based on the published data reporting the incubation period of COVID-19, the mean time between exposure and onset of clinical symptoms depended on the statistical model used, and the 95th percentile depended on the mean age of the patients. It is advisable to record sex and age when collecting data in order to analyze possible differential patterns.

[Incubation period of COVID-19: A systematic review and meta-analysis]. / Período de incubación de la COVID-19: revisión sistemática y metaanálisis.

BACKGROUND AND OBJECTIVE: The incubation period of COVID-19 helps to determine the optimal duration of the quarantine and inform predictive models of incidence curves. Several emerging studies have produced varying results; this systematic review aims to provide a more accurate estimate of the incubation period of COVID-19. METHODS: For this systematic review, a literature search was conducted using Pubmed, Scopus/EMBASE, and the Cochrane Library databases, covering all observational and experimental studies reporting the incubation period and published from 1 January 2020 to 21 March 2020.We estimated the mean and 95th percentile of the incubation period using meta-analysis, taking into account between-study heterogeneity, and the analysis with moderator variables. RESULTS: We included seven studies (n = 792) in the meta-analysis. The heterogeneity (I2 83.0%, p < 0.001) was significantly decreased when we included the study quality and the statistical model used as moderator variables (I2 15%). The mean incubation period ranged from 5.6 (95% CI: 5.2 to 6.0) to 6.7 days (95% CI: 6.0 to 7.4) according to the statistical model. The 95th percentile was 12.5 days when the mean age of patients was 60 years, increasing 1 day for every 10 years. CONCLUSION: Based on the published data reporting the incubation period of COVID-19, the mean time between exposure and onset of clinical symptoms depended on the statistical model used, and the 95th percentile depended on the mean age of the patients. It is advisable to record sex and age when collecting data in order to analyze possible differential patterns.